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It has been previously shown that in HeLa cells, HeLa S-100 factor induces a specific increase in the activity of pro-thymidine kinase (pro-TK) activity (K.F. Bromberg and S.M. Levy, Mol. Cell. Biol. 4:963, 1984). However, little is known about the regulation of the pro-TK gene. To gain further information about the regulation of this gene, the present study examined the effect of cAMP-elevating agents on the expression of pro-TK mRNA. Pro-TK mRNA level in HeLa cells is inversely proportional to the extracellular cAMP concentration. The cAMP-elevating agents, forskolin and cholera toxin, increase the expression of the pro-TK gene, and this effect is mimicked by cAMP analogs. However, the increase in the level of pro-TK mRNA by the agents is blocked by protein synthesis inhibitors. Furthermore, the in vitro transcriptional analysis demonstrated that cAMP-elevating agents stimulate the transcription of the pro-TK gene in a cAMP-dependent manner. Our studies, therefore, demonstrate that pro-TK gene expression in HeLa cells is regulated at the level of transcription by cAMP-elevating agents.Operation Little V: the first surgically produced recumbent racehorse.
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